D. Serteyn, T. Franck, P. Neven, A. Mouithys-Mickalad, C. Sandersen
Centre de l’Oxygène
Recherche et Développement (CORD)
Université de Liège, B6a
4000 Sart Tilman, Belgium
cord@ulg.ac.be
Background:
Recurrent airway obstruction (RAO) is achronic respiratory disease in horses. It is characterized byneutrophilic inflammation and delayed neutrophilic apoptosis.Curcumin has inhibitory effects on neutrophilic migration andmyeloperoxidase (MPO) release and induces neutrophilic apoptosis (1).
Aim: To assess the effects of inhalation of a hydrosoluble formof curcumin derivate, NDS27 (2), in a horse model of LPS inducedlung neutrophilia.
Method:
Eight adult horses without any evidence of respiratory disease have been used.
Neutrophil migration to the alveolar space was induced by the inhalation of 2 mg LPS in 4 ml of saline solution. Horses were nebulized 3 times with 4 mg NDS27 diluted in 4 ml saline (treated group) or with 4 ml saline (control group): 1 hour before stimulation by LPS, 1 hour and 3 hours after stimulation.
In blood samples and broncho-alveolar lavage fluids (BALF) drawned 6 hours after stimulation, cytokines (IL1, IL6, TNFalpha), MPO and elastase (ELT) were measured by ELISA.
In BALF, the percentages of neutrophils and the MPO activity were also measured (3). Statistical analysis was performed by a T test on paired data to compare the mean values (+/- SEM) with significant level at P<0.05.
Result:
In blood, IL6 (474 +/- 142 pg/ml) and MPO (91 +/- 15 ng/ml) values were significantly lower in the treated group compared to the control values of IL6 (672 +/- 199 pg/ml) and MPO (227+/- 49 ng/ml).
In BALF, total MPO (216 +/- 37 ng/ml), active MPO (8 +/- 2 ng/ml), ELT (6 +/- 1 ng/ml) and neutrophils (43 +/- 5 %) values were significantly lower in the treated group compared to the control values of total MPO (588 +/- 102 ng/ml), active MPO (30 +/- 8 ng/ml), ELT (27 +/- 5 ng/ml) and neutrophils (54 +/- 6%) values.
Variations of TNF-alpha and IL1 values were not significant (data not shown).
Conclusion:
Overall, our findings clearly indicate that NDS27 reduces the systemic and alveolar activation of neutrophils induced by LPS inhalation.
